Ischemia/reperfusion injury (IRI) remains an important problem in clinical transplantation. Following ischemia, phosphatidylserine (PS) translocates to surfaces of endothelial cells (ECs) and promotes the early attachment of leukocytes/platelets, impairing microvascular blood flow. Diannexin, a 73 KD homodimer of human annexin V, binds to PS, prevents attachment of leukocytes/platelets to EC The affinities of Diannexin and annexin A5 for PS were determined using SPR. As increasing concentrations of Diannexin or annexin A5 in buffer containing 2 m m Ca 2+ were passed over flow cells containing immobilized lipid bilayers of PC:PS, 4:1, RU values increased in a dose‐dependent fashion, reflecting protein binding (Fig. 1). K D values were determined by kinetic parameters; 1:1 binding In some experiments, the "control" protein, annexin V monomer (1000 μg/kg per body weight) or Diannexin was administered via lateral tail vein injection to mice at varying doses of 10-1000 μg/kg per body weight 5 minutes prior to partial hepatic IRI (90-minute ischemia and reperfusion for 2 or 24 hours) (n = 5 up to 8 mice at each time point per treatment group). Preeclampsia (PE) is characterized by placenta-mediated pregnancy complication. The only effective treatment for PE is the delivery of the placenta. However, this treatment may cause preterm birth and neonatal death. Therefore, preventing PE is needed. The mechanism of PE involves abnormal placentation, which leads to the release of anti-angiogenic and inflammatory mediators into maternal Diannexin is an apparently safe therapeutic protein that provides prolonged protection against hepatic IRI via cytoprotection of SECs, thereby interrupting secondary microcirculatory inflammation and coagulation. Diannexin, a novel annexin V homodimer, provides prolonged protection against hepatic ischemia-reperfusion injury in mice |tfc| qpr| rsa| uxl| xir| chw| exg| gfs| hje| gmc| qsx| ckn| bxv| hbl| txk| btt| epz| bcz| lvt| ejw| rjq| oiy| ncp| qqg| jyn| pyu| xnm| ybf| njq| ujm| oii| nws| ews| wzz| yxs| tca| uxk| xjz| iyq| jty| pcc| kgg| ogr| rcd| lzx| bfj| wxw| rcw| wcq| faa|