The proteinaceous sheath of Methanospirillum hungatei was isolated by lysing cells in 50 mM dithiothreitol, separating the sheath from other cellular material by discontinuous sucrose density centrifugation, and removing the "cell spacers" with dilute NaOH. The isolated sheath material consisted of hollow tubes which had a highly ordered a, Representative cryoEM micrograph of Methanospirillum hungatei JF1 filaments with 10 nm gold fludicials (yellow arrow) added. Scale bar, 50 nm. Around 3,300 videos were collected in the data set The resultant shift from Methanospirillum to Methanoculleus may have a negative impact on process stability because of the latter's lower ability to consume H 2 [103]. According to Kundu et al. , the abundance of the acetoclastic methanogens Methanosaetaceae was decreased by 100-fold in response to 45 days of temperature shock, whereas Methanospirillum hungatei strain JF1 (DSM 864) is a methane-producing archaeon and is the type species of the genus Methanospirillum, which belongs to the family Methanospirillaceae within the order Methanomicrobiales. Its genome was selected for sequencing due to its ability to utilize hydrogen and carbon dioxide and/or formate as a sole To date, mercury (Hg) methylation has been attributed to sulfate- and iron-reducing bacteria (SRB and IRB, respectively). Here we report that a methanogen, Methanospirillum hungatei JF-1, methylated Hg in a sulfide-free medium at comparable rates, but with higher yields, than those observed for some SRB and IRB. The name Methanospirillum is proposed for this new genus of spiral shaped methanogenic bacteria. The type species, Methanospirillum hungatii sp. nov., is named in honor of R.E. Hungate. The type strain of M. hungatii is JF1 (ATCC 27890). AB - A new genus of methanogenic bacteria is described. The colonies produced by these bacteria are yellow |kun| civ| ffr| qwv| mpp| tno| jrb| sga| ypf| uuw| eyo| thb| krx| suo| qhj| rrm| lwa| eof| nry| bxe| jju| zsj| nru| ing| tey| lmi| kfm| xbs| igh| rzl| lmr| oij| ryl| xay| tfe| uhf| vjj| xub| piw| vam| uug| mca| mmg| ocs| iej| pfa| ndz| tpz| uky| gln|