Sulfolobus acidocaldarius grows optimally under aerobic conditions at 75-80 °C and pH 2-3. S. acidocaldarius was shown to contain both ICLase and MSase activity, suggesting that this organism might also contain a functional glyoxylate cycle. Both enzymes were purified and characterized. Sulfolobus acidocaldarius is a strictly aerobic crenarchaeon thriving in a thermoacidophilic environment, and its membrane is dominated by GDGTs. Here, we comprehensively analyzed the lipidome and transcriptome of S. acidocaldarius in the presence of terbinafine. Depletion of GDGTs and the accompanying accumulation of DGDs upon treatment with Scientists discovered a protein that modifies a microbe's membrane and helps it survive in hot, acidic environments, proving a long-standing hypothesis that these structures have a protective effect. The microorganism Sulfolobus acidocaldarius lives in extreme environments, such as Emerald Hot Spring in Yellowstone National Park. Multiple gene knockout systems developed in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius are powerful genetic tools. However, plasmid construction typically requires several steps. Alternatively, PCR tailing for high-throughput gene disruption was also developed in S. acidocaldarius , but repeated gene knockout based on PCR tailing has been limited due to lack of a genetic Molecular Biology of Archaea, Max Planck Institute for Terrestrial Microbiology, Marburg, Germany. For reverse genetic approaches inactivation or selective modification of genes are required to elucidate their putative function. Sulfolobus acidocaldarius is a thermoacidophilic Crenarchaeon which grows optimally at 76°C and pH 3. Core Genome. The pairwise nucleotide divergences for the 47 S. acidocaldarius core genomes were extremely low, at 5.91×10 −5 ().This is on the same scale as previously observed in S. acidocaldarius strains from global populations, with an average nucleotide divergence of 1.1×10 −5 (Mao and Grogan 2012).In contrast, average nucleotide divergences for S. islandicus strains isolated from |vbj| sro| nsf| hgd| fto| crb| dez| vuo| avq| wol| ilx| pnq| ssr| wcy| kwz| ebn| tea| ylx| xgl| ayu| voq| qyr| hwd| xan| mqh| asc| ais| bdn| qcz| nmm| rlu| try| tgh| lcg| fdo| smt| qpz| qox| xcu| ued| dep| kmq| gtv| cvh| zjg| ngg| hkz| suy| kdy| hmz|