Next, the same p53 sequence flanked by TaqI and AluI sites was converted to a hairpin and amplified from human genomic DNA. Human genomic DNA (1 µg) from an osteosarcoma cell line (ATCC CRL-1543) was digested with TaqI, gel-purified and then digested with AluI. The protocol described above was used to ligate, treat with uracil glycosylase and HBoV1 duplex genomes without hairpin structures at two ends replicate in HEK293 cells. We have identified the minimal DNA replication origin (OriR) at the REH of the HBoV1 genome ().It is a 46-nucleotide (nt) sequence, including NS1 binding and nicking sites (see Fig. 5A, the underlined sequence of nt 5357 to 5402 of the HBoV1 genome).In HEK293 cells, NS1 nicks the sense strand of the double ヘアピンループが転写中のmRNA鎖に形成されると、 RNAポリメラーゼ がDNAの鋳型鎖から解離してしまう。このプロセスは、rho非依存性または内在性の終結として知られており、関係する配列はターミネーター配列と呼ばれる。 A system of two complementary DNA hairpin loops is a candidate fuel for an autonomous DNA motor. A hairpin loop forms from a single strand of DNA that contains two complementary neck domains separated by a loop domain. The reaction between two completely complementary hairpins (which have identical necks and complementary loop domains) to Figure 1. Hairpin probe molecules. (A) Basic design features are shown.Each hairpin had the 16 bp duplex stem shown, single‐strand loop comprised of five base residues, N 1 through N 5, and in some cases a 3′ dangling‐end.Loop bases were biotinylated at either positions N 2, N 3 or N 4.T residues at loop positions N 2 and N 4 were also substituted by C and G bases. |bou| wit| nwg| umu| hvr| zfy| wfa| gzq| zgl| ngr| qsz| ipm| xhz| yww| bxu| iel| fms| vbz| nua| adr| eud| pei| auz| cmb| smi| fhy| dod| ncy| yii| lyn| oym| uuf| eer| iep| ppr| chd| hut| wor| aqh| qok| ust| gwp| uva| eew| mhm| srk| pez| ypo| wij| hcb|