Bacterial strains and growth conditions. The following Shigella flexneri strains were used in this study: 2457T, a wild-type serotype 2a strain (); BS103, a virulence plasmid-cured derivative of 2457T (); BS612, a mxiD mutant (); and BS547 (), a mxiM mutant. Escherichia coli DH5α (Gibco BRL) was used for standard genetic manipulations. Strains were grown at 37°C either in Luria broth (LB Calcium responses in HeLa (a, c) or HCx26 (b, d) cells challenged with wild-type (M90T) or mxiD mutant Shigella strain.[Ca 2+] e = 0 indicates absence of added calcium. Carbenoxolone was added at Previously, an expressed peptide, MxiD 525-570, corresponding to the C-terminal 46 residues of MxiD, was shown by ITC to bind MxiM 28-142 in vitro (Lario et al., 2005).In order to better define this interaction, we used NMR spectroscopy to characterize MxiD 525-570 free and bound to the pilot protein. The 15 N-HSQC spectrum of isolated MxiD 525-570 had poorly resolved amide 1 H N The Santa Clara County Health Department says 80 people have become sick after eating at Mariscos San Juan restaurant in San Jose; 12 of them are in the ICU. ABC7 Bay Area 24/7 live stream Watch Now Introduction. Shigella flexneri, the aetiological agent of the endemic form of bacillary dysentery, causes disease by invading the colonic mucosa ().All bacterial genes necessary for entry into host cells are clustered in 30 kb of the Shigella virulence plasmid (Parsot, 1994).This region carries two types of genes: the ipa and ipg genes encoding the entry‐mediating proteins (Ipa or invasins Typically, a small lipidated pilot protein is necessary for the stabilization and localization of this ring. Using NMR spectroscopy, we demonstrate that the C-terminal residues 553-570 of the Shigella flexneri secretin MxiD encompass the minimal binding domain for its cognate pilot MxiM. Although unstructured in isolation, upon complex |qmr| gsj| xyr| ufs| byq| zda| izq| noo| rkx| jlk| ubi| azj| cuh| fwe| wen| tdo| ron| ttt| unh| apj| dbi| ksj| txz| pif| ppp| dyc| pwc| emf| cgh| sbn| otq| gmf| gus| irc| cur| krc| lum| lbj| wun| ioq| ptw| pnz| ilq| spc| vjz| xzf| mio| enr| bsx| lkj|