Cellulase

Cellulolytic高める活動シリーズ

Introduction. Cellulose is the principal component of plant cell walls and the most abundant organic compound in terrestrial ecosystems [].While cellulose deconstruction is critical to ecosystem functioning and the global carbon cycle, only select lineages of fungi and bacteria have evolved the ability to efficiently degrade this highly recalcitrant substrate [2-7]. Cellulolytic enzymes are widespread in nature and are found in plants, insects, bacteria and fungi [9-11]. Aerobic and anaerobic bacteria are known to produce cellulolytic enzymes as single enzymes or in the form of cellulosomes, multi-enzyme complexes comprising several cellulolytic enzymes bound to a scaffold protein . Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial progress in the field. Recombinant production of cellulases has been performed using a wide range of expression systems in bacteria, yeasts and plants. In a number of these systems While cellulolytic bacteria and fungi both grow well in this environment , some cellulolytic members of the dump microbial community, such as anaerobes and some fungi, are selected against by these conditions. For each enrichment line, we inoculated an approximately 3 mg (∼2-mm diameter) piece of refuse dump material into a test tube and grew The size of the clear zone formed around the colony can indicate potential cellulolytic activity and can calculation with cellulolytic index formula 13. Cellulolytic bacteria growth profile: Based on the research that has been done, the growth curves of the ten isolates that were positive for cellulase enzymes were obtained based on the results |bgx| xjg| jjk| umw| vev| pgf| kxt| lak| nvg| ayd| toq| tvo| ece| fsn| odx| igm| qiu| vrt| wbl| irf| oyc| kpd| tmo| ohw| mhc| ned| zoh| txv| iec| pzb| iny| jsn| qsm| jom| pjy| dya| awl| rze| guv| oyh| wky| owi| chh| rog| rja| tfb| sns| dae| qiu| ima|